If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO320
Recent progress of this line F2 individuals have been indentified
Gene name zgc:112426
Ensembl ID ENSDARG00000056504
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
Cas9 target site 2 sequence
Position in the gene exon 5
Restriction enzyme (RE) T7 endonuclease
PCR primer (forward) ccacctccaactcaaacctg
PCR primer (reverse) gtccttcagccattgatgttg
Length of expected PCR product (bp) 594
Length of expected RE digestion product (bp) 281+313
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 320
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 20
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO320.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 50%
Germline mutations in selected F0
 (By screening F1 embryos)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CGATCAGATGCxxxxxxxxxxxxxxxxCAGTGGG (-16 bp)
F0-2 CGATCAGxxxxxxxxxxxxxxxxxxxxxxxxxTGGG (-23bp)
Allele name and predicted product 320a
CGATCAGATGCxxxxxxxxxxxxxxxxCAGTGGG Truncated protein (127 aa)
CGATCAGxxxxxxxxxxxxxxxxxxxxxxxxxTGGG Truncated protein (126 aa)
填表时间 2013/12/18
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information: