China Zebrafish Resource Center (CZRC)

Product	Note	CZRC Catalog ID
pCS2+	pCS2+ for preparation of in vitro transcribed mRNA, or overexpression constructs for DNA injection and/or transfection.	CZP1
pXT7(T7:hCas9-UTRglobin)	For in vitro transcription of humanized Cas9 mRNA (CRISPR/Cas9)	CZP2
pMD19-T-gRNA scaffold	For making in vitro transcribed gRNA (CRISPR/Cas9)	CZP3
pTol2(kop:Cre-UTRnanos3,CMV:eGFP-UTRsv40)	PGC-specific Cre expression; whole embryo EGFP.	CZP5
pTol2(kop:KalTA4-UTRnanos3,CMV:eGFP-UTRsv40)	PGC-specific KalTA4 expression; whole embryo EGFP.	CZP6
pTol2(kop:loxP-sv40-loxP-mRFP-UTRnanos3,CMV:eGFP-UTRsv40)	a loxP flanked SV40 poly(A) signal between the kop promoter and the mRFP gene to regulate PGC-specific mRFP expression; whole embryo EGFP	CZP7
pTol2(UAS:mRFP-UTRnanos3,CMV:eGFP-UTRsv40)	mRFP regulated by UAS and nanos3 UTR ; whole embryo EGFP	CZP8
pTol2(kop:egfp-UTRnanos3)	PGC-specific eGFP expression	CZP9
pTol2-MCS	Tol2 Transposable Element and multi-clone sites	CZP10
pT3TS(T3:zCas9-UTRglobin)	For in vitro transcription of zebrafish codon-optimized version Cas9 mRNA (CRISPR/Cas9)	CZP11

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