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pCS2+
pCS2+ for preparation of in vitro transcribed mRNA, or overexpression constructs for DNA injection and/or transfection.
CZP1
pXT7(T7:hCas9-UTRglobin)
For in vitro transcription of humanized Cas9 mRNA (CRISPR/Cas9)
CZP2
pMD19-T-gRNA scaffold
For making in vitro transcribed gRNA (CRISPR/Cas9)
CZP3
pTol2(kop:Cre-UTRnanos3,CMV:eGFP-UTRsv40)
PGC-specific Cre expression; whole embryo EGFP.
CZP5
pTol2(kop:KalTA4-UTRnanos3,CMV:eGFP-UTRsv40)
PGC-specific KalTA4 expression; whole embryo EGFP.
CZP6
pTol2(kop:loxP-sv40-loxP-mRFP-UTRnanos3,CMV:eGFP-UTRsv40)
a loxP flanked SV40 poly(A) signal between the kop promoter and the mRFP gene to regulate PGC-specific mRFP expression; whole embryo EGFP
CZP7
pTol2(UAS:mRFP-UTRnanos3,CMV:eGFP-UTRsv40)
mRFP regulated by UAS and nanos3 UTR ; whole embryo EGFP
CZP8
pTol2(kop:egfp-UTRnanos3)
PGC-specific eGFP expression
CZP9
pTol2-MCS
Tol2 Transposable Element and multi-clone sites
CZP10
pT3TS(T3:zCas9-UTRglobin)
For in vitro transcription of zebrafish codon-optimized version Cas9 mRNA (CRISPR/Cas9)
CZP11
CZP1
CZP2
CZP3
CZP5
CZP6
CZP7
CZP8
CZP9
CZP10
CZP11
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