China Zebrafish Resource Center (CZRC)

If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn

ZKO number		ZKO875
Recent progress of this line		F2 individuals have been indentified
Gene name		ddx39aa
Ensembl ID		ENSDARG00000006225
Zebrafish strain		AB
Cas9 target site 1 sequence		antisense strand GGAAGGCCAGTTCTCGTGTGtgg
Cas9 target site 2 sequence
Position in the gene		Exon 5
Restriction enzyme (RE)		BauI (C︱ACGAG)
PCR primer (forward)		TGTGGCTAGACTGAAATTGGGT
PCR primer (reverse)		TACCCAACTGCTCCAGCATC
Length of expected PCR product (bp)		413
Length of expected RE digestion product (bp)		156+257
Plasmids used for making Cas9 mRNA		pGH-T7-zCAS9
Plasmids used for making gRNA		pMD19T-gRNA
Primers used for making gRNA		Pr-ZKO 875
Dosage of injected Cas9 mRNA (ng/ul)		300
Dosage of injected gRNA (ng/ul)		30
Survival rate of F0 embryo @ 1dpf (%)		56
Method to estimate mutation efficiency		Restriction enzyme digestionBau I
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO875.pdf
Mutation efficiency in F0 embryos (%)		6
Picture from F₀ embryos (gel picture or sequencing picture)
Mutation type		Indel
Germline transmission efficiency in adult F₀		2.7%
Wild-type (WT) sequence		CCACACACGAGAACTGGCCTTCC
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1	CCACATAGTCTGCCACGAGAACTGGCCTTCC(8bp insertion)
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	875a 875b	Truncated protein (144aa)
Allele name and predicted product	875a 875b
突变创建时间		2013-10-23
填表时间		2014/3/19
修改时间		2016/8/8
PI/负责人 (工作单位)

Genotype Information: