If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1137
Recent progress of this line F2 individuals have been indentified
Gene name dnajb1b
Ensembl ID ENSDARG00000041394
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAAAAGTTCAAGGAGATCG
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) 5’-GGCAGGAGCATAAAAGACCG-3’
PCR primer (reverse) 5’-AGAAAACAAACACGAACACAATAGT-3’
Length of expected PCR product (bp) 515
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1137
Dosage of injected Cas9 mRNA (ng/ul) 150
Dosage of injected gRNA (ng/ul) 130
Survival rate of F0 embryo @ 1dpf (%) 65
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1137.pdf
Mutation efficiency in F0 embryos (%) 10
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 50% (4/8)
Wild-type (WT) sequence CGAGGAAAAGTTCAAGGAGATCGCGGAAGC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CGAGGAAAAGTTCAAGGAGA-------AGC ( -7,+0)
F0-2 CGAGGAAAAGTTCAAGGAGA--------GC(-8, +0)
Allele name and predicted product 1137a
1137b
CGAGGAAAAGTTCAAGGAGA-------AGC Truncated protein (52 aa)
CGAGGAAAAGTTCAAGGAGA--------GC Truncated protein (53 aa)
突变创建时间 2013-10-10
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: