China Zebrafish Resource Center (CZRC)

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Commercial:¥2600(Adults)/Unit ¥1600(Embryos)/Unit ¥6000(Frozen sperm)/Unit
Non-commercial:¥1300(Adults)/Unit ¥800(Embryos)/Unit ¥3000(Frozen sperm)/Unit
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Number:

CZRZ Catalog ID:	CZ401
Availability:	Frozen sperm
Genotype:	gbf1^tsu3994/+
Affected Gene:	gbf1
Genotyping:	pdf

GENOTYPE COMPOSITION

Genomic Feature	Zygosity	Parental Genotype	Lab of Origin	Construct
^tsu3994	heterozygous		Meng Lab

Background: AB Affectedgeneurl: unspecified Linetype: Mutant Previous Names: unspecified Background: AB Affectedgeneurl: unspecified Linetype: Mutant Previous Names: unspecified

Note:

tsu3994 mutant embryos carry a T to G single nucleotide substitution in the 23rd exon. This T to G mutation is predicted to cause a leucine to arginine substitution (L1246R for X5 isoform of Gbf1) in Gbf1 protein, which is highly conserved across animal species from Caenorhabditis elegans to human. According to the NCBI database, zebrafish gbf1 may produce five transcription variants (X1– X5), which all encode an identical protein except for a few amino acids in the non-conserved linker regions. The full-length Gbf1 X5 isoform (WT) consists of 1846 residues and the L1246R mutation resides in the highly conserved HDS2 domain.

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