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ZKO number ZKO1167
Recent progress of this line F2 individuals have been indentified
Gene name CABZ01070637.1
Ensembl ID ENSDARG00000069222
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAGCAGGAAAGAGCGCATCagg
Cas9 target site 2 sequence
Position in the gene EXON 2
Restriction enzyme (RE) n/a
PCR primer (forward) AGCAATGATTTCCCGTGGGT
PCR primer (reverse) ATCGGAGAGCCCTACTGTGT
Length of expected PCR product (bp) 425
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1167
Dosage of injected Cas9 mRNA (ng/ul) 600
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%)
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1167.pdf
Mutation efficiency in F0 embryos (%) 90
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 30%
Wild-type (WT) sequence AATAAGAATATTATGCTATTGGGAGTGACTGGAGCAGGAAAGAGCGCATCAGGAAACACCATCTTGGGTGAAAACAAATTTAC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 AATAAGAATATTATGCTATTGGGAGTGACTGGAGCAGGAAAGAGGAAACACCATCAGGAAACACCATCTTGGGTGAAAACAAATTTAC
F0-2 AATAAGAATATTATGCTATTGGGAGTGACTGGAGCAGGAAA---------------CACCATCTTGGGTGAAAACAAATTTAC
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 AATAAGAATATTATGCTATTGGGAGTGACTGGAGCAGGAAAGAGGAAACACCATCAGGAAACACCATCTTGGGTGAAAACAAATTTAC
F0-2 AATAAGAATATTATGCTATTGGGAGTGACTGGAGCAGGAAA---------------CACCATCTTGGGTGAAAACAAATTTAC
Allele name and predicted product 1167a
1167b
ATGTCGAATAAGAATATTATGCTATTGGGAGTGACTGGAGCAGGAAAGAGGAAACACCATCAGGAAACACCATCTTGGGTGAAAACAAATTTACAGTAA truncated protein (32aa)
突变创建时间 2015-03-15
填表时间 2013/12/20
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information: