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ZKO number ZKO1162
Recent progress of this line F1 progeny has been raised
Gene name CU280887.1
Ensembl ID ENSDARG00000081157
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGTTGGATCATTTACTAGTG
Cas9 target site 2 sequence
Position in the gene intron
Restriction enzyme (RE) n/a
PCR primer (forward) GGTCGTTCATGTTGCTCTCG
PCR primer (reverse) CATTAGAATGACCGTGTAGG
Length of expected PCR product (bp) 300
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1162
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency PCR-SSCP detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1162.pdf
Mutation efficiency in F0 embryos (%) 5
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 1/50
Wild-type (WT) sequence
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCATCCATTCCCTCACCGGTATACCAATGCAAGAAGGCCTTGCGGCGAAACATAGCCGTGAACTGCTCTGAAATTCGCTTGAAGAGCTCCTGGATGGCTGTACTGTTTCCAATGAAGGTCGCCGACATTTTGAGCCCGCGAGGTGGAATGTCGCAGACGGCCGTCTTGACGTTATTGTGAGGATGAACTGAAAATGCTCAGGTAACGATTAATGACAGGTACATTTTTTCCTACACGGTCATTCTAATG
F0-2 GGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCATCCATTCCCTCACCGGTATACCAATGCAAGAAGGCCTTGCGGCGAAACATAGCCGTGAACTGCTCTGAAATTCGCTTGAAGAGCTCCTGGATGGCTGTACTGTTTCCAATGAAGGTCGCCGACATTTTGAGCCCGCGAGGTGGAATGTCGCAGACGGCCGTCTTGACGTTATTGTGAGGATGAACTGAAAATGCTCAGGTAACGATTAATGACAGGTACATTTTTTCCTACACGGTCATTCTAATG
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCATCCATTCCCTCACCGGTATACCAATGCAAGAAGGCCTTGCGGCGAAACATAGCCGTGAACTGCTCTGAAATTCGCTTGAAGAGCTCCTGGATGGCTGTACTGTTTCCAATGAAGGTCGCCGACATTTTGAGCCCGCGAGGTGGAATGTCGCAGACGGCCGTCTTGACGTTATTGTGAGGATGAACTGAAAATGCTCAGGTAACGATTAATGACAGGTACATTTTTTCCTACACGGTCATTCTAATG
F0-2 GGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCATCCATTCCCTCACCGGTATACCAATGCAAGAAGGCCTTGCGGCGAAACATAGCCGTGAACTGCTCTGAAATTCGCTTGAAGAGCTCCTGGATGGCTGTACTGTTTCCAATGAAGGTCGCCGACATTTTGAGCCCGCGAGGTGGAATGTCGCAGACGGCCGTCTTGACGTTATTGTGAGGATGAACTGAAAATGCTCAGGTAACGATTAATGACAGGTACATTTTTTCCTACACGGTCATTCTAATG
Allele name and predicted product 1162a
1162b
突变创建时间
填表时间 2013/12/18
修改时间 2016/12/30
PI/负责人 (工作单位)
Genotype Information: