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ZKO number ZKO93
Recent progress of this line F1 progeny has been raised
Gene name ndfip2
Ensembl ID ENSDARG00000033928
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GATTCCCTCCAAAGCGCCAAcgg
Cas9 target site 2 sequence
Position in the gene exon 2
Restriction enzyme (RE) BstX 1
PCR primer (forward) GTTGCACAGTGAGGATGACT
PCR primer (reverse) TTATCAAGACAATAACCACAT
Length of expected PCR product (bp) 459
Length of expected RE digestion product (bp) 231+228
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 93
Dosage of injected Cas9 mRNA (ng/ul) 1000
Dosage of injected gRNA (ng/ul) 300
Survival rate of F0 embryo @ 1dpf (%) 30
Method to estimate mutation efficiency Restriction enzyme digestionBstX 1
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO93.pdf
Mutation efficiency in F0 embryos (%) 20
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 30
Wild-type (WT) sequence CCGCCAGTGCTGAACCGTCCACATCCACCCAGGCCGTTGGCGCTTTGGAGGGAATCAGCAACCTAGCAGTGGATACAGAAGC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 CCGCCAGTGCTGAACCGTCCACATCCACCCAGGCCGTTGG----------AGAATCAGCAACCTAGCAGTGGATACAGAAGC (+1bp,-11bp)
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 20% CCGCCAGTGCTGAACCGTCCACATCCACCCAGGCCGTTGG----------AGAATCAGCAACCTAGCAGTGGATACAGAAGC (+1bp,-11bp)
F0-2
Allele name and predicted product 93a
93b
CCGCCAGTGCTGAACCGTCCACATCCACCCAGGCCGTTGG----------AGAATCAGCAACCTAGCAGTGGATACAGAAGC, Truncated protein (40aa)
突变创建时间 2015-06-18
填表时间 2013/12/18
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information: