If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO968
Recent progress of this line F2 individuals have been indentified
Gene name sgms2
Ensembl ID ENSDARG00000052520
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGGCAGGTTGCACCCGTGGAtgg
Cas9 target site 2 sequence
Position in the gene Exon 4
Restriction enzyme (RE) n/a
PCR primer (forward) 5'-ACATCCTTTAGGAGCTGAATC-3'
PCR primer (reverse) 5'-GAACGTCTTCCACCATTCC-3'
Length of expected PCR product (bp) 336
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids used for making gRNA pT7-gRNA-ZKOpT7-gRNA-ZKO968
Primers used for making gRNA Pr-ZKO 968
Dosage of injected Cas9 mRNA (ng/ul) 200
Dosage of injected gRNA (ng/ul) 25
Survival rate of F0 embryo @ 1dpf (%) 70
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO968.pdf
Mutation efficiency in F0 embryos (%) 33
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 50% (2/4)
Wild-type (WT) sequence AAACACTTCCCGAACAGATGGAGCTCCATCCACGGGTGCAACCTGCCCCGTCCACTCAGCCGAAGACGCAAAG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 AAACACTTCCCGAACAGATGGAGCTCCATC-------------TGCCCCGTCCACTCAGCCGAAGACGCAAAG
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 AAACACTTCCCGAACAGATGGAGCTCCATC-------------TGCCCCGTCCACTCAGCCGAAGACGCAAAG
F0-2
Allele name and predicted product 968a
968b
AAACACTTCCCGAACAGATGGAGCTCCATC-------------TGCCCCGTCCACTCAGCCGAAGACGCAAAG Truncated protein (36aa)
突变创建时间 2014-06-13
填表时间 2013/12/18
修改时间 2016/9/21
PI/负责人 (工作单位)
Genotype Information: