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ZKO number ZKO996
Recent progress of this line F2 individuals have been indentified
Gene name kif16ba
Ensembl ID ENSDARG00000074158
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGGAAGTCACACACCATGATggg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) n/a
PCR primer (forward) GCACTGACACATTAACCCATGC
PCR primer (reverse) GTAGCAAGTCATGACTCAGAGC
Length of expected PCR product (bp) 338
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pT7-gRNA-ZKOpT7-gRNA-ZKO996
Primers used for making gRNA Pr-ZKO 996
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 80
Survival rate of F0 embryo @ 1dpf (%) 90
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO996.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 30
Wild-type (WT) sequence TTTGCTTATGGTCAGACTGGATCAGGGAAGTCACACACCATGATGGGAATCCCTgtaagttctttctgtttaaacttttcgcgagctgaatattggctctgagtcatgacttgctac
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 TTTGCTGATGGGAATCCCTgtaagttctttctgtttaaacttttcgcgagctgaatattggctctgagtcatgacttgctac(-35bp)
F0-2 TTTGCTTATGGTCAGACTGGATCAGGGAAGTCACACACCATTGATGGGAATCCCTgtaagttctttctgtttaaacttttcgcgagctgaatattggctctgagtcatgacttgctac(+1bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TTTGCTGATGGGAATCCCTgtaagttctttctgtttaaacttttcgcgagctgaatattggctctgagtcatgacttgctac(-35bp)
F0-2 TTTGCTTATGGTCAGACTGGATCAGGGAAGTCACACACCATTGATGGGAATCCCTgtaagttctttctgtttaaacttttcgcgagctgaatattggctctgagtcatgacttgctac(+1bp)
Allele name and predicted product 996a
996b
TTTGC...................................TGATGGGAATCCCT
TTTGCTTATGGTCAGACTGGATCAGGGAAGTCACACACCATTGATGGGAATCCCT
突变创建时间
填表时间 2013/12/18
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: