If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO188
Recent progress of this line F1 individuals have been identified
Gene name gng13a
Ensembl ID ENSDARG00000024740
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GCTCCTAAACCTCAGTGTCagg,GCCATGAAGGACAACCCGTggg
Cas9 target site 2 sequence
Position in the gene 5'UTR-exon3
Restriction enzyme (RE) n/a
PCR primer (forward) ACCGATTCTCCCTGGCAAAG
PCR primer (reverse) TGCTGTTGTATAGTTGTCTTACACT
Length of expected PCR product (bp) 1096
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 188
Dosage of injected Cas9 mRNA (ng/ul) 600
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 85
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO188.pdf
Mutation efficiency in F0 embryos (%) 10
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 3%
Wild-type (WT) sequence GCTCCTAAACCTCAGTGTCAGG<672bp>GCCATGAAGGACAACCCGTGGG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 3%(1/31) AAAGCAGTTTGAATGCTCCTAAACCTCAGT <-694bp> CGTGGGTGGAGTCGAGCAAATGTGTAATAC (-694bp)
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 2%(2/97) AAAGCAGTTTGAATGCTCCTAAACCTCAGT <-694bp> CGTGGGTGGAGTCGAGCAAATGTGTAATAC (-694bp)
F0-2
Allele name and predicted product 188a
188b
AAAGCAGTTTGAATGCTCCTAAACCTCAGT<-694bp>CGTGGGTGGAGTCGAGCAAATGTGTAATAC;Truncated protein(null)
突变创建时间 2013-12-13
填表时间 2013/12/18
修改时间 2016/12/30
PI/负责人 (工作单位)
Genotype Information: