If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO183
Recent progress of this line F0 individuals have been indentified
Gene name zgc:152698
Ensembl ID ENSDARG00000058464
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GAGGGTCAGTGGGAAATTTTcgg,GCAGAAGAACACAAGTTGACtgg
Cas9 target site 2 sequence
Position in the gene 5'UTR~3'UTR
Restriction enzyme (RE) n/a
PCR primer (forward) ACCGGCACTTTGAGTCTCTG
PCR primer (reverse) GAAAACCCCTCTCCGCCTTT
Length of expected PCR product (bp) 1572
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 183
Dosage of injected Cas9 mRNA (ng/ul) 600
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO183.pdf
Mutation efficiency in F0 embryos (%) 9
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 10%
Wild-type (WT) sequence ctcaaccgtttgcgcaaattgcgacccgaaa <1119bp> gaacttgtgttcttctgcttttaatgaaatt
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 ctcaaccgtttgcgcaaattgcgacccgaaagaacttgtgttcttctgcttttaatgaaatt (-1119bp)
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 ctcaaccgtttgcgcaaattgcgacccgaaagaacttgtgttcttctgcttttaatgaaatt (-1119bp)
F0-2
Allele name and predicted product 183a
183b
ctcaaccgtttgcgcaaattgcgacccgaaagaacttgtgttcttctgcttttaatgaaatt
突变创建时间 2013-12-05
填表时间 2013/12/18
修改时间 2016/12/30
PI/负责人 (工作单位)
Genotype Information: