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ZKO number ZKO61
Recent progress of this line F1 individuals have been identified
Gene name atp1a1a.5
Ensembl ID ENSDARG00000040252
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GTGGCGGCGACTTCAGAAGAtgg
Cas9 target site 2 sequence
Position in the gene Exon2
Restriction enzyme (RE) n/a
PCR primer (forward) GATGCTGCTGGATTCATCAT
PCR primer (reverse) TTCACGAATACTTTCATACC
Length of expected PCR product (bp) 255
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 61
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 150
Survival rate of F0 embryo @ 1dpf (%) 95
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO61.pdf
Mutation efficiency in F0 embryos (%) 50
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 50
Wild-type (WT) sequence ACAGGGAAAGATGACTATAAAGTGGCGGCGACTTCAGAAGATGGAGTCAAAACGCCTAAAAAAGGGAAAAAGA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 ACAGGGAAAGATGACTATAAAGTGGCGGCGACTT........GGAGTCAAAACGCCTAAAAAAGGGAAAAAGAACAAAAAGGACATGG(-8bp)
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 ACAGGGAAAGATGACTATAAAGTGGCGGCGACTT........GGAGTCAAAACGCCTAAAAAAGGGAAAAAGAACAAAAAGGACATGG(-8bp)
F0-2
Allele name and predicted product 61a
61b
ACAGGGAAAGATGACTATAAAGTGGCGGCGACTT........GGAGTCAAAACGCCTAAAAAAGGGAAAAAGAACAAAAAGGACATGG Truncated protein(1013aa)
突变创建时间 2015-05-18
填表时间 2013/12/18
修改时间 2016/8/19
PI/负责人 (工作单位)
Genotype Information: