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ZKO number ZKO182
Recent progress of this line F1 individuals have been identified
Gene name palb2
Ensembl ID ENSDARG00000076716
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGGCTTCGCTTGCATGCAGggg,GGATTAATACTCTCCAGTTGggg
Cas9 target site 2 sequence
Position in the gene Exon4~5
Restriction enzyme (RE) n/a
PCR primer (forward) AGCAGTTTCCTTACAACTGGACA
PCR primer (reverse) CCTGCATCAGAGAATGGCGT
Length of expected PCR product (bp) 2916
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 182
Dosage of injected Cas9 mRNA (ng/ul) 600
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO182.pdf
Mutation efficiency in F0 embryos (%) 8
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 1.5%
Wild-type (WT) sequence CCCCTGCATGCAAGCGAAGCCC<2358bp>CCCCAACTGGAGAGTATTAATCC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 AGCAGTTTCCTTACAACTGGACATATGAACAGAAAAAAAACAACCCTTGGACAATAAGAAAAAAAAGTGTTTTTATCCAGATTTAGGTGTAGTGCTAAAGTTAAAGTGTGTTCAAATTACATAAAGCCTATTTAAAATTCTAATCAATTCTGAATACTAACAAACATTGGTTTTCATTTTGCTCTCCAGACAGAAACATCTCAGATTGTTCTGCTGTTGAGAAAACCCCTGCCTTCCAATTGCAACTAGCTGAGATTACATCTACACCGTCTTTGTACTCCCCT <-2382bp> TTAATCCTGTTCAAAATTGTGAAAGCTCTAAGCTTTGGTCTGAAACCCAAAGTCAAAGCCATTATAAATGAAAAAACATTTAATTGTGGGAATGAATTTAGTTCTGAATGTCAAACTCCTTTTGATAAGACCTCTTTGGAGACTTCTGTAAATGAGAGTACAGAAAGAAGGAATCCGTCAGAGGAATTTTCTTCCAACAATCTGACATTATTTCATGAGCAAACGCCATTCTCTGATGCAGG
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 1.5%(1/65) AGCAGTTTCCTTACAACTGGACATATGAACAGAAAAAAAACAACCCTTGGACAATAAGAAAAAAAAGTGTTTTTATCCAGATTTAGGTGTAGTGCTAAAGTTAAAGTGTGTTCAAATTACATAAAGCCTATTTAAAATTCTAATCAATTCTGAATACTAACAAACATTGGTTTTCATTTTGCTCTCCAGACAGAAACATCTCAGATTGTTCTGCTGTTGAGAAAACCCCTGCCTTCCAATTGCAACTAGCTGAGATTACATCTACACCGTCTTTGTACTCCCCT <-2382bp> TTAATCCTGTTCAAAATTGTGAAAGCTCTAAGCTTTGGTCTGAAACCCAAAGTCAAAGCCATTATAAATGAAAAAACATTTAATTGTGGGAATGAATTTAGTTCTGAATGTCAAACTCCTTTTGATAAGACCTCTTTGGAGACTTCTGTAAATGAGAGTACAGAAAGAAGGAATCCGTCAGAGGAATTTTCTTCCAACAATCTGACATTATTTCATGAGCAAACGCCATTCTCTGATGCAGG
F0-2
Allele name and predicted product 182a
182b
TTACATCTACACCGTCTTTGTACTCCCCTG<-2382bp>GGAGAGTATTAATCCTGTTCAAAATTGTGA; Truncated protein(136aa)
突变创建时间 2013-11-05
填表时间 2013/12/18
修改时间 2016/12/30
PI/负责人 (工作单位)
Genotype Information: