If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO215
Recent progress of this line F2 generation has been raised
Gene name zgc:194355
Ensembl ID ENSDARG00000077652
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGCTACACACCTTGTCCATAtgg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) n/a
PCR primer (forward) CGTGGCTCATCTTATTCCTG
PCR primer (reverse) TCATGCTAGTGCCGTATCTG
Length of expected PCR product (bp) 317
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 215
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 30
Survival rate of F0 embryo @ 1dpf (%) 85
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO215.pdf
Mutation efficiency in F0 embryos (%) 10
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence GATTCTGGTTAACCATATGGACAAGGTG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GATTCTGGTTAACCATAT-GACAAGGTG
F0-2 GATTCTGGTTAACCAT--GGTTAACAAGGTG
Allele name and predicted product 215a
215b
GATTCTGGTTAACCATAT-GACAAGGTG truncated protein (162aa)
GATTCTGGTTAACCAT--GGTTAACAAGGTG truncated protein (116aa)
突变创建时间 2013-05-10
填表时间 2013/12/18
修改时间 2016/10/14
PI/负责人 (工作单位)
Genotype Information: