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ZKO number ZKO209
Recent progress of this line F2 generation has been raised
Gene name trim2b
Ensembl ID ENSDARG00000076174
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGACGCTCTTCCTGGTGTCGagg
Cas9 target site 2 sequence
Position in the gene exon3
Restriction enzyme (RE) n/a
PCR primer (forward) TACTGGAGATGTTTCTGTGACT
PCR primer (reverse) AATGACATTGCTAGAACTTTG
Length of expected PCR product (bp) 367
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 209
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 60
Survival rate of F0 embryo @ 1dpf (%) 75
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO209.pdf
Mutation efficiency in F0 embryos (%) 20
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence CAAAACCCTCGACACCAGGAAGAGCGTCCTGCTGA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CAAAACCCTC-----------GAGCGTCCTGCTGA
F0-2 CAAAACCCTCGA----AGAGCGAAGAGCGTCCTGCTGA
Allele name and predicted product 209a
209b
CAAAACCCTC-----------GAGCGTCCTGCTGA truncated protein (182aa)
CAAAACCCTCGA----AGAGCGAAGAGCGTCCTGCTGA truncated protein (134aa)
突变创建时间 2013-05-17
填表时间 2013/12/18
修改时间 2016/10/14
PI/负责人 (工作单位)
Genotype Information: