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ZKO number ZKO207
Recent progress of this line F2 generation has been raised
Gene name si:dkeyp-75b4.9
Ensembl ID ENSDARG00000015678
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGCAGTCTAAGAGAGTGAGAtgg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) n/a
PCR primer (forward) GCATGCTTATTGTTTTATGTTC
PCR primer (reverse) TTCATTTAATCTACCACAGTGTG
Length of expected PCR product (bp) 268
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 207
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 30
Survival rate of F0 embryo @ 1dpf (%) 85
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO207.pdf
Mutation efficiency in F0 embryos (%) 15
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence CTAGAACAACACCATCTCACTCTCTTAGACTGCCTTGA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CTAGAACAACACCATCT--------------GCCTTGA
F0-2 CTAGAACAACACCATCTTAGACACTCTCTTAGACTGCCTTGA
Allele name and predicted product 207a
207b
CTAGAACAACACCATCT--------------GCCTTGA truncated protein (30aa)
CTAGAACAACACCATCTTAGACACTCTCTTAGACTGCCTTGA truncated protein (36aa)
突变创建时间 2013-05-09
填表时间 2013/12/18
修改时间 2016/10/14
PI/负责人 (工作单位)
Genotype Information: