If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO191
Recent progress of this line F2 generation has been raised
Gene name si:ch211-57e21.4
Ensembl ID ENSDARG00000092791
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGCCATATTGCTCAGACTGAcgg,GTAAGGTATAGGCTTACTGggg
Cas9 target site 2 sequence
Position in the gene flanking exon2
Restriction enzyme (RE) n/a
PCR primer (forward) ACCCGGTTTGACAGTTTGGT
PCR primer (reverse) CAATGCCGGCAAACCACATA
Length of expected PCR product (bp) 764
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 191
Dosage of injected Cas9 mRNA (ng/ul) 500
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO191.pdf
Mutation efficiency in F0 embryos (%) 20
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 2/8
Wild-type (WT) sequence GATGAACTCTTTTTGGCGCGGCCATATTGC<531bp>agcctataccttactcagtgtacccatata
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GATGAACTCTTTTTGGCGCGGCCATATTGCagcctataccttactcagtgtacccatata (-531bp)
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GATGAACTCTTTTTGGCGCGGCCATATTGCagcctataccttactcagtgtacccatata (-531bp)
F0-2
Allele name and predicted product 191a
191b
GATGAACTCTTTTTGGCGCGGCCATATTGC<-531bp>agcctataccttactcagtgtacccatata null
突变创建时间 2015-05-20
填表时间 2013/12/17
修改时间 2016/12/30
PI/负责人 (工作单位)
Genotype Information: