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ZKO number ZKO1025
Recent progress of this line F2 individuals have been indentified
Gene name si:dkey-121b10.7
Ensembl ID ENSDARG00000059948
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGAGCTTCTTCCCTTCCTTAggg
Cas9 target site 2 sequence
Position in the gene exon2
Restriction enzyme (RE) HpyAV
PCR primer (forward) CCTCCGTTTAGCGTGTTAT
PCR primer (reverse) CCTTGTCGTAGAACCTGTC
Length of expected PCR product (bp) 483
Length of expected RE digestion product (bp) 206=277
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1025
Dosage of injected Cas9 mRNA (ng/ul) 230
Dosage of injected gRNA (ng/ul) 70
Survival rate of F0 embryo @ 1dpf (%)
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1025.pdf
Mutation efficiency in F0 embryos (%)
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 33.3
Wild-type (WT) sequence TCAGAGCTTCCGAAGCAAACTTTTCCCTAAGGAAGGGAAGAAGCTCCAGGCGGACGCGCG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TCAGAGCTTCCGAAGCAAACTTTTCCCTAAAAGGGAAGAAGCTCCAGGCGGACGCGCG
F0-2 TCAGAGCTTCCGAAGCAAACTTTTCCCTAATAATGATGGGAAGAAGCTCCAGGCGGACGCGCG
Allele name and predicted product 1025a
1025b
突变创建时间 2013-09-05
填表时间 2013/12/17
修改时间 2016/8/9
PI/负责人 (工作单位)
Genotype Information: