China Zebrafish Resource Center (CZRC)

If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn

ZKO number		ZKO123
Recent progress of this line		F2 individuals have been indentified
Gene name		pcnp
Ensembl ID		ENSDARG00000037713
Zebrafish strain		AB
Cas9 target site 1 sequence		sense strand GCATGACAGCAAAGAAACCTAtgg
Cas9 target site 2 sequence
Position in the gene		Exon 2
Restriction enzyme (RE)		Msc I (TGG/CCA)
PCR primer (forward)		5’- gtagctcacctttggtctcc -3’…………47.8
PCR primer (reverse)		5’- ggcagcagcttaatcaaag -3’…………47.8
Length of expected PCR product (bp)		499
Length of expected RE digestion product (bp)		245 bp +254 bp
Plasmids used for making Cas9 mRNA		pXT7-hCAS9
Plasmids used for making gRNA		pMD19T-gRNA
Primers used for making gRNA		Pr-ZKO 123
Dosage of injected Cas9 mRNA (ng/ul)
Dosage of injected gRNA (ng/ul)
Survival rate of F0 embryo @ 1dpf (%)
Method to estimate mutation efficiency		sequencing of PCR product
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO123.pdf
Mutation efficiency in F0 embryos (%)
Picture from F₀ embryos (gel picture or sequencing picture)
Mutation type		Indel
Germline transmission efficiency in adult F₀
Wild-type (WT) sequence
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	123a 123b
Allele name and predicted product	123a 123b
突变创建时间
填表时间		2013/12/17
修改时间		2016/12/29
PI/负责人 (工作单位)

Genotype Information: