If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO123
Recent progress of this line F2 individuals have been indentified
Gene name pcnp
Ensembl ID ENSDARG00000037713
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) Msc I (TGG/CCA)
PCR primer (forward) 5’- gtagctcacctttggtctcc -3’…………47.8
PCR primer (reverse) 5’- ggcagcagcttaatcaaag -3’…………47.8
Length of expected PCR product (bp) 499
Length of expected RE digestion product (bp) 245 bp +254 bp
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 123
Dosage of injected Cas9 mRNA (ng/ul)
Dosage of injected gRNA (ng/ul)
Survival rate of F0 embryo @ 1dpf (%)
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO123.pdf
Mutation efficiency in F0 embryos (%)
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence
Germline mutations in selected F0
 (By screening F1 embryos)
Mosaicism in selected F0
 (By screening F1 adults)
Allele name and predicted product 123a
填表时间 2013/12/17
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information: