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ZKO number ZKO110
Recent progress of this line F2 individuals have been indentified
Gene name gtf3c3
Ensembl ID ENSDARG00000043247
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGTGGCGATGGTGAGGGCTCagg
Cas9 target site 2 sequence
Position in the gene exon2
Restriction enzyme (RE) BanII
PCR primer (forward) ctgtgtatctgtccaccagca
PCR primer (reverse) TTTCCTTTAGGGGCCACATGA
Length of expected PCR product (bp) 484
Length of expected RE digestion product (bp) 130+350
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 110
Dosage of injected Cas9 mRNA (ng/ul) 500
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency Restriction enzyme digestionBanII digestion
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO110.pdf
Mutation efficiency in F0 embryos (%) 40
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 30%
Wild-type (WT) sequence TTTGTGATAGAGTGCTGTTTGTTTTGTACAGGTGGCGATGGTGAGGGCTCAGGTGTGTGTTGTTGCTGACATGTCCTCGGGTGTCAAA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 TTTGTGATAGAGTGCTGTTTGTTTTGTACAGGTGGCGATGGTGAGGG------TGTGTGTTGTTGCTGACATGTCCTCGGGTGTCAAA (-6bp)
F0-2 TTTGTGATAGAGTGCTGTTTGTTTTGTACAGGTGGCGATGGTGAGGG------TGTGTGTTGTTGCTGACATGTCctcgggtgtcaaaCTCGGGTGTCAAA (+7bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TTTGTGATAGAGTGCTGTTTGTTTTGTACAGGTGGCGATGGTGAGGG------TGTGTGTTGTTGCTGACATGTCctcgggtgtcaaaCTCGGGTGTCAAA (+7bp) (+7bp)
F0-2 TTTGTGATAGAGTGCTGTTTGTTTTGTACAGGTGGCGATGGTGAGGG------TGTGTGTTGTTGCTGACATGTCCTCGGGTGTCAAA (-6bp)
Allele name and predicted product 110a
110b
TTTGTGATAGAGTGCTGTTTGTTTTGTACAGGTGGCGATGGTGAGGGTGTGTGTTGTTGCTGACATGTCCTCGGGTGTCAAACTCGGGTGTCAAA truncated protein(610aa)
突变创建时间
填表时间 2013/12/17
修改时间 2016/12/30
PI/负责人 (工作单位)
Genotype Information: