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ZKO number ZKO980
Recent progress of this line F2 generation has been raised
Gene name si:ch73-190m4.1
Ensembl ID ENSDARG00000075785
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGTAAAGACAGTGACCAAGA
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) n/a
PCR primer (forward) GAGACGCTGCTGAAGTCTGA
PCR primer (reverse) TGTACAGTACTACTACAGCCGG
Length of expected PCR product (bp) 550
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 980
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 20
Survival rate of F0 embryo @ 1dpf (%) 45
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO980.pdf
Mutation efficiency in F0 embryos (%) 35
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 100*(1/6)
Wild-type (WT) sequence CTGCGCGGTAAAGACAGTGACCAAGATGGAAAA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 CTGCGCGGTAAAGACAGTGACAGTGAGATGGAAAA
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CTGCGCGGTAAAGACAGTGACAGTGAGATGGAAAA
F0-2
Allele name and predicted product 980a
980b
CTGCGCGGTAAAGACAGTGACAGTGAGATGGAAAA Truncated protein (67aa)
突变创建时间 2014-04-16
填表时间 2013/12/16
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information: