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ZKO number ZKO103
Recent progress of this line F2 individuals have been indentified
Gene name si:ch1073-345a8.1
Ensembl ID ENSDARG00000092671
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGCTGTAGTAGCTCTTCAGAGagg
Cas9 target site 2 sequence
Position in the gene exon1
Restriction enzyme (RE) Hpy188I (TC/NAG)
PCR primer (forward) 5’- agcttcataaagcaggccgt -3’
PCR primer (reverse) 5’- CCTTACACGGGACGACAACTC -3’
Length of expected PCR product (bp) 409
Length of expected RE digestion product (bp) 230bp +179 bp
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 103
Dosage of injected Cas9 mRNA (ng/ul) 1000
Dosage of injected gRNA (ng/ul) 300
Survival rate of F0 embryo @ 1dpf (%) 60
Method to estimate mutation efficiency Restriction enzyme digestion60
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO103.pdf
Mutation efficiency in F0 embryos (%) 60
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 60
Wild-type (WT) sequence GGTTTACTCGAGGCTGTAGTAGCTCTTCAGAGA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GGTTTACTCGAGGCTGTAGTAGCTCTTCA-AGAGGAGGA (+0 bp, -1 bp)
F0-2 GGTTTACTCGAGGCTGTAG-----------AGAGGAGGA (+0 bp, -11 bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GGTTTACTCGAGGCTGTAG-----------AGAGGAGGA (+0 bp, -11 bp)
F0-2 GGTTTACTCGAGGCTGTAGTAGCTCTTCA-AGAGGAGGA (+0 bp, -1 bp)
Allele name and predicted product 103a
103b
(+0bp, -1 bp)
(+0bp, -11 bp)
突变创建时间
填表时间 2013/12/16
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information: