If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO212
Recent progress of this line F2 generation has been raised
Gene name fga
Ensembl ID ENSDARG00000020741
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
TGATAACAGGTATATGTCTCtgg
Cas9 target site 2 sequence
Position in the gene exon4
Restriction enzyme (RE) n/a
PCR primer (forward) GTGTTTGTCAGCAACAAGGGAACG
PCR primer (reverse) GATGTGAATGGTTTCAGGTTTACAGC
Length of expected PCR product (bp) 455
Length of expected RE digestion product (bp) 185+270
Plasmids used for making Cas9 mRNA pGH-T7-zCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 212
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 20
Survival rate of F0 embryo @ 1dpf (%) 70
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO212.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 20
Wild-type (WT) sequence TGATAACAGGTATATGTCTCTGGCCGAACAACT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TGATAACAGGTATATGT----GGCCGAACAACT(-4bp)
F0-2
Allele name and predicted product 212a
212b
TGATAACAGGTATATGT----GGCCGAACAACT;Truncated protein (128aa)
突变创建时间 2015-06-10
填表时间 2013/12/16
修改时间 2017/1/3
PI/负责人 (工作单位)
Genotype Information: