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ZKO number ZKO1192
Recent progress of this line F2 individuals have been indentified
Gene name METRNL
Ensembl ID ENSDARG00000007289
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GTGCTGAAGGCTCTCTGCAGtgg
Cas9 target site 2 sequence
Position in the gene exon 2
Restriction enzyme (RE) PstI
PCR primer (forward) tatgaggaaatgtctgatgt
PCR primer (reverse) AGACTGAAGCAGCGCACGCGT
Length of expected PCR product (bp) 426
Length of expected RE digestion product (bp) 231+195
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1192
Dosage of injected Cas9 mRNA (ng/ul) 500
Dosage of injected gRNA (ng/ul) 200
Survival rate of F0 embryo @ 1dpf (%) 70
Method to estimate mutation efficiency Restriction enzyme digestionPstI
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1192.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 30
Wild-type (WT) sequence CTGACGCACGAGTCTCACGCGCGGGATGTGGAGCAGGTGTATCTGCGCTGTGCTGAAGGCTCTCTGCAGTGGCTGTATCCCACCGGAGCGGTGATGGTGAACCTGCGGCCGAACCTGCCGTCCT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 CTGACGCACGAGTCTCACGCGCGGGATGTGGAGCAGGTGTATCTGCGCTGTGCTGAAGGCTCTCT--CAGTGGCTGTATCCCACCGGAGCGGTGATGGTGAACCTGCGGCCGAACCTGCCGTCCT
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CTGACGCACGAGTCTCACGCGCGGGATGTGGAGCAGGTGTATCTGCGCTGTGCTGAAGGCTCTCT--CAGTGGCTGTATCCCACCGGAGCGGTGATGGTGAACCTGCGGCCGAACCTGCCGTCCT
F0-2
Allele name and predicted product 1192a
1192b
CTGACGCACGAGTCTCACGCGCGGGATGTGGAGCAGGTGTATCTGCGCTGTGCTGAAGGCTCTCT--CAGTGGCTGTATCCCACCGGAGCGGTGATGGTGAACCTGCGGCCGAACCTGCCGTCCT (+0bp, -1bp) truncated protein 59aa
突变创建时间
填表时间 2014/9/18
修改时间 2016/12/29
PI/负责人 (工作单位)
Genotype Information: