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ZKO number ZKO1173
Recent progress of this line F1 individuals have been identified
Gene name CABZ01059404.1
Ensembl ID ENSDARG00000088906
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GATCGCACTGCACCGTTATCagg
Cas9 target site 2 sequence
Position in the gene exon4
Restriction enzyme (RE) n/a
PCR primer (forward) GGATgtacgtactaatcttgtttac
PCR primer (reverse) ccacccattccagtatatatgt
Length of expected PCR product (bp) 264
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1173
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1173.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 40%
Wild-type (WT) sequence TCCATCTCGCAGACGGCCTCAACAGCCGTGCCACTGGATTCAGATCGCACTGCACCGTTATCAGGTAATGTTTCTCATGTGAAATAATGATGCACAAATAATTTCAAACATATATACTGGAATGGGTGG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TCCATCTCGCAGACGGCCTCAACAGCGGTGCCACTGGATTCGGATCAGGTAATGTTTCTCATGTGAAATAATGATGCACAAATAATTCCAAACATATATACTGGAATGGGTGG(-16bp)
F0-2 TCCATCTCGCAGACGGCCTCAACAGCGGTGCCACTGGATTCGGATTGCACTGCACCGTAATGTTTCTCATGTGAAATAATGATGCACAAATAATTCCAAACATATATACTGGAATGGGTGG(-8bp)
Allele name and predicted product 1173a
1173b
TCCATCTCGCAGACGGCCTCAACAGCGGTGCCACTGGATTCGGATCAGGTAATGTTTCTCATGTGAAATAATGATGCACAAATAATTCCAAACATATATACTGGAATGGGTGG; truncated protein (311aa)
TCCATCTCGCAGACGGCCTCAACAGCGGTGCCACTGGATTCGGATTGCACTGCACCGTAATGTTTCTCATGTGAAATAATGATGCACAAATAATTCCAAACATATATACTGGAATGGGTGG;(change splicing site)
突变创建时间 2015-07-10
填表时间 2014/3/28
修改时间 2016/10/14
PI/负责人 (工作单位)
Genotype Information: