If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1175
Recent progress of this line F1 individuals have been identified
Gene name CABZ01059408.1
Ensembl ID ENSDARG00000059786
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAGGATCAGCTACTATATggg
Cas9 target site 2 sequence
Position in the gene exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) TTCTGCTGGGTAAAACCG
PCR primer (reverse) TGCTATTCTCGCATAGTGTCT
Length of expected PCR product (bp) 641
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1175
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 40
Survival rate of F0 embryo @ 1dpf (%) 60
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1175.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0
Wild-type (WT) sequence CGTCATTCTGTTCACTCATGCTGATGCACTGGAGGATCAGCTACTATATGGGTATATCAGTCAAAGTGGTGATCTCTGG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CGTCATTCTGTTCACTCATGCTGATGCACTGGAGGATCAGCTACTAtgggTATGGGTATATCAGTCAAAGTGGTGATCTCTGG; (+4bp)
F0-2 CATCATTCTGTTCACTCATGCTGATGCACTGGAGGATCAGTTATattgttgtTATATGGGTATATCAGTCAAAGTGGTGATCTCTGG;(-4bp; +12bp)
Allele name and predicted product 1175a
1175b
CGTCATTCTGTTCACTCATGCTGATGCACTGGAGGATCAGCTACTAtgggTATGGGTATATCAGTCAAAGTGGTGATCTCTGG; truncated protein (147aa)
CATCATTCTGTTCACTCATGCTGATGCACTGGAGGATCAGTTATattgttgtTATATGGGTATATCAGTCAAAGTGGTGATCTCTGG; truncated protein (169aa)
突变创建时间 2014-09-02
填表时间 2014/3/28
修改时间 2016/10/14
PI/负责人 (工作单位)
Genotype Information: