If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1064
Recent progress of this line F2 generation has been raised
Gene name xpo1a
Ensembl ID ENSDARG00000078041
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GAAGGACCATCCAGATGCC
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) BstXI (CCANNNNN/NTGG)
PCR primer (forward) CCGTTGCCTTGAGTGTTCAG
PCR primer (reverse) TCATGTTCTGCGAGAACTCCAA
Length of expected PCR product (bp) 384
Length of expected RE digestion product (bp) 341+43
Plasmids used for making Cas9 mRNA pSP6-2sNLS-spCas9
Plasmids used for making gRNA pT7-gRNA-ZKO011
Primers used for making gRNA Pr-ZKO 1064
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 200
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency Restriction enzyme digestionBstXI
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1064.pdf
Mutation efficiency in F0 embryos (%) 50
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 70% (7/10)
Wild-type (WT) sequence CCTGAAGGACCATCCAGATGCCTGGACCAG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CCTGAAGGACCATCCAGATGCATGCCTGGACCAG
F0-2 CC---------------ACGCCTGGACCAG
Allele name and predicted product 1064a
1064b
CCTGAAGGACCATCC-------TGGACCAG
CC---------------ACGCCTGGACCAG
突变创建时间 2013-05-13
填表时间 2014/1/4
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information: