If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1063
Recent progress of this line F2 generation has been raised
Gene name usp34
Ensembl ID ENSDARG00000037734
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GAGAACTGCGCCGAGCTGC
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) CATCAAGCATTTCGTCGGGCC
PCR primer (reverse) GCAAACAACTACAAACAGGG
Length of expected PCR product (bp) 214
Length of expected RE digestion product (bp) N/A
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1063
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 200
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1063.pdf
Mutation efficiency in F0 embryos (%) 50
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 37.5% (6/16)
Wild-type (WT) sequence TGTGAGAACTGCGCCGAGCTGCTGGAGGTG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TGTGAGAACTGCGCC---------GAGGTG
F0-2 TGTGAGAACTGCGCCGAGtgtgagaaCTGCTGGAGGTG
Allele name and predicted product 1063a
1063b
TGTGAGAACTGCGCC---------GAGGTG
TGTGAGAACTGCGCCGAGtgtgagaaCTGCTGGAGGTG
突变创建时间 2013-05-03
填表时间 2014/1/4
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information: