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ZKO number ZKO1061
Recent progress of this line F1 individuals have been identified
Gene name si:ch211-213b8.4
Ensembl ID ENSDARG00000095360
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGTCACTTTATTATGCATAG
Cas9 target site 2 sequence
Position in the gene Exon 8
Restriction enzyme (RE) n/a
PCR primer (forward) CCTCTCCAGGTGATGACTGC
PCR primer (reverse) CCTGAGAGACAGCCCAACAG
Length of expected PCR product (bp) 870
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 30
Survival rate of F0 embryo @ 1dpf (%) 40
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1061.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 20% (3/16)
Wild-type (WT) sequence CCGCTATGCATAATAAAGTG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CCGCTAT----AATAAAGTG
F0-2 CCGCTA ATAATACCGCATAATAAATAATACC GCATAATAAA
Allele name and predicted product 1061a
1061b
CCGCTAT----AATAAAGTG Truncated protein
CCGCTA ATAATACCGCATAATAAATAATACC GCATAATAA Extended protein
突变创建时间 2014-05-25
填表时间 2014/1/4
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information: