If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1057
Recent progress of this line F1 individuals have been identified
Gene name elmod2
Ensembl ID ENSDARG00000052370
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GAAACACACAGACGCAGCC
Cas9 target site 2 sequence
Position in the gene Exon 7
Restriction enzyme (RE) Hga1 (GACGC R︱R)
PCR primer (forward) TCCCCATTCGACTGTCTAGGT
PCR primer (reverse) TGTTGTTTGAGTGAACCTCACA
Length of expected PCR product (bp) 613
Length of expected RE digestion product (bp) 225+388
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1057
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 91.6
Method to estimate mutation efficiency Restriction enzyme digestionHga1
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1057.pdf
Mutation efficiency in F0 embryos (%) 20
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 25% (8/32)
Wild-type (WT) sequence GAAACACACAGACGCAGCCCGGCAGGTTC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 GAAACACACAGACG-------GCAGGTTC (-7bp) GAAACACACAGACGCAG--------GTTC (-8bp)
F0-2 GAAACACACAGACG-----CGGCAGGTTC (-5bp) GAAACACACAGACGCAAGCCCGCGGGGTTC (+1bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GAAACACACAGACG-------GCAGGTTC (-7bp)
F0-2 GAAACACACAGACGCAG--------GTTC (-8bp)
Allele name and predicted product 1057a
1057b
GAAACACACAGACG-------GCAGGTTC;(-7bp) ; Truncated protein (232 aa)
GAAACACACAGACGCAG--------GTTC;(-8bp) ; Truncated protein (250 aa)
突变创建时间 2014-01-14
填表时间 2014/1/4
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information: