If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1047
Recent progress of this line F1 individuals have been identified
Gene name wu:fc75a09
Ensembl ID ENSDARG00000089342
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
gagagaccgctgcgaaactg tgg
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) n/a
PCR primer (forward) ctcctgaagagcatccaatc
PCR primer (reverse) caaaaggattttcagagtatgg
Length of expected PCR product (bp) 245
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1047
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 30
Survival rate of F0 embryo @ 1dpf (%) 75
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1047.pdf
Mutation efficiency in F0 embryos (%) 50
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 40% (2/5)
Wild-type (WT) sequence gtgagagaccgctgcgaaactgtggctgcaccatactctg
Germline mutations in selected F0
 (By screening F1 embryos)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 gtgagagaccgctgcgaa--tgtggctgcaccatactctg
F0-2 gtgagagaccgctgc--------------accatactctg
Allele name and predicted product 1047a
gtgagagaccgctgcgaa--tgtggctgcaccatactctgTruncated protein (39aa)
gtgagagaccgctgc--------------accatactctg Truncated protein (265 aa)
突变创建时间 2014-03-19
填表时间 2014/1/3
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information: