If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO979
Recent progress of this line F1 individuals have been identified
Gene name ppm1k
Ensembl ID ENSDARG00000010655
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGTGGACAAAGCATTAGCG
Cas9 target site 2 sequence
Position in the gene Exon 3
Restriction enzyme (RE) n/a
PCR primer (forward) ccaacatctcaaccatttggcag
PCR primer (reverse) cgcaaacctccatttggctctg
Length of expected PCR product (bp) 419
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO
Dosage of injected Cas9 mRNA (ng/ul) 320
Dosage of injected gRNA (ng/ul) 120
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO979.pdf
Mutation efficiency in F0 embryos (%) 50
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 40% (2/5)
Wild-type (WT) sequence gaggtggacaaagcattagcgaggcatctt
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 acaaagcattaagcgaggcatccttca
F0-2 acaaagca---gcgaggcatcttca
Allele name and predicted product 979a
979b
Acaaagcattaagcgaggcatccttca(+1bp)
acaaagca---gcgaggcatcttca(-3bp)
突变创建时间 2014-08-01
填表时间 2014/1/2
修改时间 2016/11/11
PI/负责人 (工作单位)
Genotype Information: