ZKO number ZKO14
Recent progress of this line F0 population has been raised
Gene name gas6
Ensembl ID ENSDARG00000007804
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
5’- GGTCATTAAGATTGACAGGG agg -3’
Cas9 target site 2 sequence
Position in the gene Exon 11
Restriction enzyme (RE) T7EI
PCR primer (forward) 5’- TGGCATATCAGTCAAAATAGGAC -3’
PCR primer (reverse) 5’- CCACCGTCAGGTTCAGCTC -3’
Length of expected PCR product (bp) 305
Length of expected RE digestion product (bp) 210,90
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 14
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 60
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO14.pdf
Mutation efficiency in F0 embryos (%) 10
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 10%
Wild-type (WT) sequence 5'--GCGGAGCCTGGTCATTAAGATTGACAGGGAGGCGGTGATG--3'
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 5'--GCGGAGCCTGGTCATTAAGATTG----GGAGGCGGTGATG--3'
F0-2 5'--GCGGAGCCTGGTCATTAAGATTGA--------------ATG--3'
Allele name and predicted product 14a
14b
5'--GCGGAGCCTGGTCATTAAGATTG----GGAGGCGGTGATG--3' Trancated protein (387 aa)
5'--GCGGAGCCTGGTCATTAAGATTGA--------------ATG--3' Trancated protein (397 aa)
突变创建时间
填表时间 2013/12/24
修改时间 2016/8/8
PI/负责人 (工作单位)
Genotye Information: