If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO6
Recent progress of this line F1 progeny has been raised
Gene name pcid2
Ensembl ID ENSDARG00000013802
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
5’- GGTGTACTCGTCCTTCAGGT tgg -3’
Cas9 target site 2 sequence
Position in the gene Exon 10
Restriction enzyme (RE) T7EI
PCR primer (forward) 5’-GACACTTTGAGTAGTGATCCAGTC-3’
PCR primer (reverse) 5’-GGGTAAAGGAGAACGACAGAC -3’
Length of expected PCR product (bp) 495
Length of expected RE digestion product (bp) 350,150
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 6
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 60
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO6.pdf
Mutation efficiency in F0 embryos (%) 20
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 10%
Wild-type (WT) sequence 5'- AAGCCGCTCATCCGCGCCATCGACAGCTCCAACCTGAAG -3'
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 5'- AAGCCGCTCATCCGCGCCATCGACAGCTCCA-----AAG -3' (-5 bp)
F0-2
Allele name and predicted product 6a
6b
5'- AAGCCGCTCATCCGCGCCATCGACAGCTCCA-----AAG -3' (-5 bp) Truncated protein 227 aa
突变创建时间
填表时间 2013/12/24
修改时间 2016/8/8
PI/负责人 (工作单位)
Genotype Information: