If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1087
Recent progress of this line F1 individuals have been identified
Gene name null
Ensembl ID ENSDARG00000030417
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGCTGTGGTGTTGGGCGTGGagg
Cas9 target site 2 sequence
Position in the gene Exon 7
Restriction enzyme (RE) n/a
PCR primer (forward) ACTCTGAGGCTGTCCTATCC
PCR primer (reverse) ACCTCTCTGCCATCCTTACT
Length of expected PCR product (bp) 343
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1087
Dosage of injected Cas9 mRNA (ng/ul) 120
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1087.pdf
Mutation efficiency in F0 embryos (%) 15
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 40%
Wild-type (WT) sequence CCCGGGTCAACACCTCCACGCCCAACACCACAGCCAGG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 CCCGGGTCAACACCTC--------------CAGCCAGG
F0-2 CCCGGGTCAACACCTCCA-GCCCAACACCACAGCCAGG
Allele name and predicted product 1087a
1087b
CCCGGGTCAACACCTC--------------CAGCCAGG Truncated protein (266aa)
CCCGGGTCAACACCTCCA-GCCCAACACCACAGCCAGG Truncated protein (212aa)
突变创建时间 2014-07-07
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: