If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1120
Recent progress of this line F2 generation has been raised
Gene name zgc:158803
Ensembl ID ENSDARG00000019765
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGCGCGTGCAGAGCAGCTAGGcgg
Cas9 target site 2 sequence
Position in the gene Exon5
Restriction enzyme (RE) n/a
PCR primer (forward) AGCCAGTATCCACTTCAATGAGT
PCR primer (reverse) GAAAGGTTTATAGGACTGCCTGTA
Length of expected PCR product (bp) 699
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1120
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 60
Method to estimate mutation efficiency sequencing of monoclone
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1120.pdf
Mutation efficiency in F0 embryos (%) 25
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 10%(2/20)
Wild-type (WT) sequence GGGAAGCTGCTGGCGCGTGCAGAGCAGCTAGGCGGCGAGGGGAACGTGGAC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GGGAAGCTGCTGGCGCGTGCAGAGCAGAGCAGGCGGCGAGGGGAACGTGGAC
F0-2
Allele name and predicted product 1120a
1120b
GGGAAGCTGCTGGCGCGTGCAGAGCAGAGCAGGCGGCGAGGGGAACGTGGAC Truncated protein (190aa)
突变创建时间 2014-05-12
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: