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ZKO number ZKO1116
Recent progress of this line F2 generation has been raised
Gene name sertad2a
Ensembl ID ENSDARG00000093412
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGACTCAGACTCGTCCAGACtgg
Cas9 target site 2 sequence
Position in the gene exon 3
Restriction enzyme (RE) n/a
PCR primer (forward) TTGCGTTCTTACTGCACTGCAAG
PCR primer (reverse) GAGCTGAAAGCGTCCTTAGTGAT
Length of expected PCR product (bp) 534
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1116
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 70
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1116.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 28.6%(10/35)
Wild-type (WT) sequence AAGTGAGTAATAATGGCTCCAGTCTGGACGAGTCTGAGTCCGAGTCCTCCAGCGGCT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 AAGTGAGTAATAATGGCTCCAGTCTGAGTCCGAGTCCTCCAGCGGCT
F0-2
Allele name and predicted product 1116a
1116b
AAGTGAGTAATAATGGCTCCAGTCTGAGTCCGAGTCCTCCAGCGGCT Truncated protein (45aa)
突变创建时间 2013-12-23
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: