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ZKO number ZKO1122
Recent progress of this line F2 individuals have been indentified
Gene name null
Ensembl ID ENSDARG00000036281
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGAGCTGGAGGCAGAGCTTAtgg
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) n/a
PCR primer (forward) CCAATACTGAGTTGCAGCTGGAAGT
PCR primer (reverse) ATACAGAGAGCTGCCATTCGCTCAAT
Length of expected PCR product (bp) 487
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1122
Dosage of injected Cas9 mRNA (ng/ul) 400
Dosage of injected gRNA (ng/ul) 200
Survival rate of F0 embryo @ 1dpf (%) 60
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1122.pdf
Mutation efficiency in F0 embryos (%) 30
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 10%(2/20)
Wild-type (WT) sequence GGAAATGAGGCGGAGCTGGAGGCAGAGCTTATGGCATTGATGGGTGGAGGAGGTGGAGGT
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GAGGAAATGAGGCGGAGCTGGAGGCAGAGCT------------GGAGGAGGTGGAGGTAGAGGCGGAGCCGG
F0-2
Allele name and predicted product 1122a
1122b
GAGGAAATGAGGCGGAGCTGGAGGCAGAGCT------------GGAGGAGGTGGAGGTAGAGGCGGAGCCGG Truncated protein (79aa)
突变创建时间 2013-12-29
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: