If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1117
Recent progress of this line F2 generation has been raised
Gene name si:ch73-131e21.5
Ensembl ID ENSDARG00000052263
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
CCATAGAGCTCGACGGGAAGacc
Cas9 target site 2 sequence
Position in the gene exon 3
Restriction enzyme (RE) n/a
PCR primer (forward) ACTGAACCCCAACTGCTCCCT
PCR primer (reverse) GGCAACCCCTGAAAAAGAGACTA
Length of expected PCR product (bp) 860
Length of expected RE digestion product (bp)
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1117
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 100
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1117.pdf
Mutation efficiency in F0 embryos (%) 12
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 14%(1/7)
Wild-type (WT) sequence TTTAAGATCAGGACCATAGAGCTCGACGGGAAGACCATTAAACTACAGATC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TTTAAGATCAGGACCATAGCTCGACGGGAAGACCATTAAACTACAGATC
F0-2
Allele name and predicted product 1117a
1117b
TTTAAGATCAGGACCATAGCTCGACGGGAAGACCATTAAACTACAGATC Truncated protein (56aa)
突变创建时间 2013-09-22
填表时间 2013/12/20
修改时间 2016/8/18
PI/负责人 (工作单位) 石德利(山东大学生命科学学院)
Genotype Information: