If you need ZKO Alleles,please contact luyuanpan@ihb.ac.cn
ZKO number ZKO1085
Recent progress of this line F2 individuals have been indentified
Gene name null
Ensembl ID ENSDARG00000042887
Zebrafish strain AB
Cas9 target site 1 sequence antisense strand
GGTTTGAGGTGGGCGTCCGGcgg
Cas9 target site 2 sequence
Position in the gene Exon 1
Restriction enzyme (RE) n/a
PCR primer (forward) CATCCAGTGTCTGTGTGATAAG
PCR primer (reverse) GAACCGTCCTCATGCCTACT
Length of expected PCR product (bp) 285
Length of expected RE digestion product (bp) n/a
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 1085
Dosage of injected Cas9 mRNA (ng/ul) 100
Dosage of injected gRNA (ng/ul) 120
Survival rate of F0 embryo @ 1dpf (%) 85
Method to estimate mutation efficiency sequencing of PCR product
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO1085.pdf
Mutation efficiency in F0 embryos (%) 45
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type Indel
Germline transmission efficiency in adult F0 75%
Wild-type (WT) sequence GGTAGTAAGCCAGCCGCCGGACGCCCACCTCAAACCGCTCA
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GGTAGTAAGCCAGCCGCCG-----------CAAACCGCTCA
F0-2 GGTAGTAA--------------GCCCACCTCAAACCGCTCA
Allele name and predicted product 1085a
1085b
GGTAGTAAGCCAGCCGCCG-----------CAAACCGCTCA Truncated protein (58aa)
GGTAGTAA--------------GCCCACCTCAAACCGCTCA Truncated protein (57aa)
突变创建时间 2013-11-02
填表时间 2013/12/20
修改时间 2016/8/11
PI/负责人 (工作单位)
Genotype Information: