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ZKO number ZKO167
Recent progress of this line F2 generation has been raised
Gene name abcg2b
Ensembl ID ENSDARG00000079361
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGGCACTCTGACTGTGCggg
Cas9 target site 2 sequence
Position in the gene 5', 4th exon
Restriction enzyme (RE) T7
PCR primer (forward) tgagccaccgtgcctccagaatg
PCR primer (reverse) caaccttggtgtctgcacagtcc
Length of expected PCR product (bp) 276
Length of expected RE digestion product (bp) 130+140
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids used for making gRNA pMD19T-gRNA
Primers used for making gRNA Pr-ZKO 167
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 25
Survival rate of F0 embryo @ 1dpf (%) 50
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO167.pdf
Mutation efficiency in F0 embryos (%) 10
Picture from F0 embryos
  (gel picture or sequencing picture)
Mutation type deletion
Germline transmission efficiency in adult F0 20
Wild-type (WT) sequence cgaaccagtgaccttcttgctgtgaggcaacagtgctaaccactgagccaccgtgcctccagaatgcattattatcattttctgttcttttgctttaggatcaaacaattggtttaatgcccggcattttctttgccctctgcttgtgtgtccatctaggatgatgtcctgatgggcactctgactgtgcgggagaatcttttattctctggaaatctgcgcttaccaagggaacagttttccagtgctgataagaaaaaaaaagtggagagcatcatcgaggagcttggactggaggactgtgcagacaccaaggttgaattcagtttattatgcatctttattttaatgtctagatataattttggtagaagcaattttaaaactgcaaatattacttaattcctttatttttgtctccaaaaatgtgactatcgtgatttcacatattgaaaatgtaactttgatcatgatcattttttgaacaaccaaccagcagccattaa
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 AGGATGATGTCCTGATGGGCACTCTGACTGAATCTTTTATTCTCTGGAAATCTGCGCTTA (-8bp)
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 AGGATGATGTCCTGATGGGCACTCTGACTGAATCTTTTATTCTCTGGAAATCTGCGCTTA (-8bp)
F0-2
Allele name and predicted product 167a
167b
truncated protein (173 aa)
突变创建时间 2013-07-19
填表时间 2013/12/20
修改时间 2016/9/23
PI/负责人 (工作单位)
Genotype Information: