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Optimal Tcf motif, TOP driving GFP expression
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CZRZ Catalog ID: CZ25
Availability: Embryos/Adults
Genotype: ihb27Tg/+
Genotyping: pdf
GENOTYPE COMPOSITION
Genomic Feature Zygosity Parental Genotype Lab of Origin Construct
ihb27Tg heterozygous  Fish Developmental Biotechnology Lab Tg(OTM:dGFP)
Phenotype:
Note:
Canonical Wnt reporter: Optimal Tcf motif, TOP driving GFP expression. The TOPdGFP reporter construct contains four consensus Lef binding sites and a minimal cFos promoter, driving a destabilized GFP transgene. This reporter should only be transcriptionally active in the presence of both stabilized beta-catenin and Lef/Tcf proteins.
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